ELISA Activity

Question 3

What would happen if the anti-human Ig-conjugate were not washed free of the well before the substrate was added?

The ELISA would not develop when the substrate was added.

The ELISA would develop normally.

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate.

Both A and B.

Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu