ELISA Activity

Question 2

What would happen if serum were omitted from the ELISA, but all other steps remained the same and were performed properly?

Anti-human Ig-conjugate would not bind and be washed away.

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate.

The O.D. values would be nearly the same as the assay control.

Both A and C

Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu