ELISA Activity

Question 3

What would happen if serum were omitted from the ELISA, but all other steps remained the same and were performed properly?

All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate.

CORRECT!
Since the enzyme which acts on the substrate is in excess, it would turn all the wells a uniform color whether they were truly positive or not.

Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu