ELISA Activity

Question 3

What would happen if serum were omitted from the ELISA, but all other steps remained the same and were performed properly?

The ELISA would develop normally.

INCORRECT.
The substrate would over-develop because an excess of enzyme coupled to the anti-human Ig was present.

Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu