ELISA Activity

Question 2

What would happen if serum were omitted from the ELISA, but all other steps remained the same and were performed properly?

Both A and C

CORRECT!
Since no serum was added, the anti-human Ig conjugate would not be able to bind to human Ig and the O.D. values would be the same as the assay control.

Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu