ELISA Activity

Question 2

What would happen if serum were omitted from the ELISA, but all other steps remained the same and were performed properly?

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate.

INCORRECT.The anti-human Ig conjugate is specific for human Ig and has very little non-specific binding activity.

Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu