INCORRECT

Question 3

When performing RAST how would one know if IgE levels are high or low in a given serum?

If the optical density of the liqid in the tubes is high, then the patient has high levels of antigen-specific IgE.
Explanation
Radioactivity is measured in a RAST, not optical density. Also, positive and negative controls with known amounts of allergen-specific IgE are necessary to quantitate the levels of IgE in a given serum.

Immunological Techniques
University of Arizona
Monday, October 6, 2008
dlake@azc.arizona.edu

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