Western Blot Activity

Question 4

Which patient(s) is/are HIV positive by Western blot?

Substrate

INCORRECT.
Incorrect. Although patient B may have antibodies directed against p24, p66 and p55, the serum contains no antibodies against HIV envelope glycoproteins, gp160, 120. Therefore this test is indeterminate for patient B. Patient C is HIV positive because the serum shows reactivity to gp160, 120, p24, 66 and 55.

Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu