Western Blot Activity

Question 2

In strip 1, the combining sites of primary antibodies are bound to:

Substrate

INCORRECT.
Second antibody or conjugate in this case is an anti-human Ig coupled to alkaline phosphatase. This antibody binds to the primary antibody. The conjugate binds to the primary antibody after the primary has bound to the antigen.

Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu