Western Blot Activity

Question 2

In strip 1, the combining sites of primary antibodies are bound to:

Substrate

INCORRECT.
Substrate is the chromogen which gets dephosphorylated by alkaline phosphatase resulting in a color change and precipitation onto the region of the membrane where the second antibody bound to the primary antibody.

Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu