ELISA Activity

	Partially purified, inactivated HIV antigens pre-coated onto an ELISA plate
	Patient serum which contains antibodies. If the patient is HIV+, then this serum will contain antibodies to HIV, and those antibodies will bind to the HIV antigens on the plate.
	Anti-human immunoglobulin coupled to an enzyme. This is the second antibody, and it binds to human antibodies.
	Chromagen or substrate which changes color when cleaved by the enzyme attached to the second antibody.

Positive ELISA Test

Animation
(3 M)
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Negative ELISA Test

Animation
(2.7 M)
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Immunological Techniques
University of Arizona
Monday, October 6, 2008
douglas.lake@asu.edu